DNA Sequencing


DNA Sequencing

DNA Sequencing by Capillary Electrophoresis is a key technology in a number of experimental and diagnostic workflows in the life science laboratory. The facility uses the 3500XL platform to deliver this service.

DNA Concentration guideline

Template Quantity/ul Primer/ul
PCR Products:100-200 bp

200-500 bp

500-1000 bp

1000-2000 bp

>2000 bp

1-3 ng

3-10 ng

5-20 ng

10-40 ng

40-1000 ng

5 pmol
Single Stranded DNA 50-100 ng 5 pmol
Double stranded DNA Plasmid 150-500 ng 5 pmol
Cosmid, BAC DNA 0.5-2.0 µg TBD
Bacterial genomic DNA 2-3 µg TBD

Please provide at least 3ul/reaction for template and primer. For subsequent analysis please visit the software page for more information.

DNA Sequencing Service Request Forms

DNA Sequencing Request Form – 16 Sample (PDF)

DNA Sequencing Request Form – 16 Sample (Word)

DNA Sequencing Request Form – 96 Sample (Excel)

 

Sequencing Primers available through service, we are happy to add other primers on request:

Name Sequence Length
M13 rev (-29) CAG GAA ACA GCT ATG ACC 18bp
M13 rev (-49) GAG CGG ATA ACA ATT TCA CAC AGG 24bp
M13 uni (-21) TGT AAA ACG ACG GCC AGT 18bp
SP6 CAT TTA GGT GAC ACT ATA G 19bp
T3 AAT TAA CCC TCA CTA AAG GG 20bp
T7 TAA TAC GAC TCA CTA TAG GG 20bp
T7 term CTA GTT ATT GCT CAG CGG T 19bp
pCR 3.1 (BGHrev.) TAG AAG GCA CAG TCG AGG 18bp
pGex for ATA GCA TGG CCT TTG CAG G 19bp
pGex rev GAG CTG CAT GTG TCA GAG G 19bp
CMV-for CGC AAA TGG GCG GTA GGC CTG 21bp
CMV-rev CCT CCA CCC CAT AAT ATT ATA GAA GGA CAC 30bp
U19 GGT TTT CCC AGT CAC GAC G 19bp