Fragment analysis using capillary electrophoresis on the Applied Biosystems 3500xL Genetic Analyser is used to provide an accurate estimate of the size of fluorescently labelled DNA fragments. Multiple DNA fragments labelled with several different fluorophores can be analysed in a single well and the size of each fragment is extrapolated from a size ladder included in each well. This technique can be used in many applications including microsatellite instability analysis, fingerprinting (e.g. AFLP) and the detection of small deletions or insertions.
The technique is robust and, depending on the assay carried out, can be inexpensive.
Please contact us with any questions or requests.