Fragment Analysis


Fragment Analysis

Fragment analysis generates a size estimate for DNA fragments relative to a size standard of DNA fragments with known lengths. The size standard is combined with the sample of interest and co-injected on the capillary electrophoresis system.

What is Fragment Analysis

  • Labeling fragments with fluorescent dyes. Multiple different colored fluorescent dyes can be detected in one sample. One of the dye colors is used for a labeled size standard present in each sample. The size standard is used to extrapolate the base-pair sizes of the sample product peaks.
  • Amplifying the labeled fragments using polymerase chain reaction (PCR) on a thermal cycler.
  • Separating the fragments by size using capillary electrophoresis.
  • Analyzing the data using software to determine:
    • Size : The analysis software uses the size standard in each sample to create a standard curve for each sample. It then determines the relative size of each dye labeled fragment in the sample by comparing fragments with the standard curve for that specific sample.
    • Genotype : The analysis software assigns allele calls based on user defined makers (loci).