NovaSeq 6000
The Illumina NovaSeq 6000 is a scalable NGS instrument with enough capacity to cost-effectively sequence medium to large scale sequencing experiments such as gene expression, transcriptomics, single-cell sequencing including libraries prepared using the 10x Chromium Controller (available in the UoD’s Flow Cytometry & Cell Sorting facility), whole exome sequencing, and whole genome sequencing of even large genomes. For example, the NovaSeq 6000 is capable of sequencing up to 48 whole human genomes in one run using two flow cells. This impressive instrument reduces the cost-per-read of NGS experiments. For details on different sequencing options for the MiSeq, please consult the table below:
| Service offered | Maximum reads per flow cell | Maximum gigabases per flow cell | Read length paired end | Read length single end |
| SP 100 cycles | 800 million | 80 Gb | 2 x 50 bp | 1 x 100 bp |
| SP 200 cycles | 800 million | 167 Gb | 2 x 100 bp | 1 x 200 bp |
| SP 300 cycles | 800 million | 250 Gb | 2 x 150 bp | 1 x 300 bp |
| SP 500 cycles | 800 million | 400 Gb | 2 x 250 bp | 1 x 500 bp |
| S1 100 cycles | 1.6 billion | 167 Gb | 2 x 50 bp | 1 x 100bp |
| S1 200 cycles | 1.6 billion | 333 Gb | 2 x 100 bp | 1 x 200 bp |
| S1 300 cycles | 1.6 billion | 500 Gb | 2 x 150 bp | 1 x 300 bp |
| S2 100 cycles | 4.1 billion | 417 Gb | 2 x 50 bp | 1 x 100 bp |
| S2 200 cycles | 4.1 billion | 833 Gb | 2 x 100 bp | 1 x 200 bp |
| S2 300 cycles | 4.1 billion | 1250 Gb | 2 x 150 bp | 1 x 300 bp |
| S4 35 cycles | 10 billion | 350 Gb | – | 1 x 35 bp |
| S4 200 cycles | 10 billion | 2000 Gb | 2 x 100 bp | 1 x 200 bp |
| S4 300 cycles | 10 billion | 3000 Gb | 2 x 150 bp | 1 x 300 bp |
MiSeq
The MiSeq is ideally suited for sequencing of targeted genotyping, 16s metagenomics, targeted expression profiling, miRNA, small RNA or whole genome sequencing of smaller genomes e.g. bacteria, fungi, viruses. The MiSeq is able to produce up to 300 base reads with a maximum of 25 million reads per run. For details on different sequencing options for the MiSeq, please consult the table below:
| Service offered | Maximum reads per flow cell | Maximum gigabases per flow cell | Read length paired end | Read length single end |
| Nano 300 cycles | 1 million | 0.3 Gb | 2 x 150 bp | 1 x 300 bp |
| Nano 500 cycles | 1 million | 0.5 Gb | 2 x 250 bp | – |
| Micro 300 cycles | 4 million | 1.2 Gb | 2 x 150 bp | 1 x 300 bp |
| 50 cycles | 15 million | 0.85 Gb | – | 1 x 50 bp |
| 150 cycles | 25 million | 3.8 Gb | 2 x 75 bp | 1 x 150bp |
| 300 cycles | 15 million | 5.1 Gb | 2 x 150 bp | 1 x 300 bp |
| 600 cycles | 25 million | 15 Gb | 2 x 300 bp | – |