Next Generation Sequencing Using Illumina MiSEQ

The TCGA operates Illumina MiSEQ for short-read next generation sequencing (NGS) on a variety of sample types.The MiSeq is ideally suited for sequencing of targeted genotyping, 16s metagenomics, targeted expression profiling, miRNA, small RNA or whole genome sequencing of smaller genomes e.g. bacteria, fungi, viruses. The MiSeq is able to produce up to 300 base reads with a maximum of 25 million reads per run. For details on different sequencing options for the MiSeq, please consult the table below:

Service offered	Maximum reads per flow cell	Maximum gigabases per flow cell	Read length paired end	Read length single end
Nano 300 cycles	1 million	0.3 Gb	2 x 150 bp	1 x 300 bp
Nano 500 cycles	1 million	0.5 Gb	2 x 250 bp	–
Micro 300 cycles	4 million	1.2 Gb	2 x 150 bp	1 x 300 bp
50 cycles	15 million	0.85 Gb	–	1 x 50 bp
150 cycles	25 million	3.8 Gb	2 x 75 bp	1 x 150bp
300 cycles	15 million	5.1 Gb	2 x 150 bp	1 x 300 bp
600 cycles	25 million	15 Gb	2 x 300 bp	–