Next Generation Sequencing


The TCGA operates Illumina MiSeq and NextSeq instruments for Next Generation Sequencing (NGS) on a variety of sample types and for a range of applications including targeted genotyping, 16s metagenomics, gene expression, transcriptomics, miRNA and small RNA analysis, epigenetics and whole genome sequencing.

Our standard NGS service includes library QC, sequencing of user-prepared libraries and QC of the data. For this standard service please complete the NGS Request Form and send it to tcga@dundee.ac.uk.

A more comprehensive service, including library construction, can be arranged by special request, please contact the laboratory for a quote.

MiSeq

The MiSeq is ideally suited for sequencing of targeted genotyping, 16s metagenomics, targeted expression profiling, miRNA, small RNA or whole genome sequencing of smaller genomes e.g. bacteria, fungi, viruses. The MiSeq is able to produce up to 300 base reads with a maximum of 25 million reads per run. For details on different library options for the MiSeq, please consult the table below:

Service offered Maximum clusters per flow cell Maximum gigabases per flow cell Read length paired end Read length single end
Nano 300 cycles 1 million 0.3 Gb 2 x 150 bp 1 x 300 bp
Nano 500 cycles 1 million 0.5 Gb 2 x 250 bp
Micro 300 cycles 4 million 1.2 Gb 2 x 150 bp 1 x 300 bp
50 cycles 15 million 0.85 Gb 1 x 50 bp
150 cycles 25 million 3.8 Gb 2 x 75 bp 1 x 150bp
300 cycles 15 million 5.1 Gb 2 x 150 bp 1 x 300 bp
600 cycles 25 million 15 Gb 2 x 300 bp

NextSeq 500

The NextSeq 500 allows mid to high-throughput sequencing in a benchtop instrument and is capable of analysing targeted genotyping, gene expression, transcriptome, exome and whole genome sequencing. The NextSeq is able to produce up to 150 base reads with a maximum 400 million (high output) reads per run. For details on different library options for the NextSeq, please consult the table below:

Service offered Maximum clusters per flow cell Maximum gigabases per flow cell Read length (paired end) Read length (single end)
Mid output, 150 cycles 130 million 19.5 Gb 2x 75 bp 1x 150 bp
Mid output, 300 cycles 130 million 39 Gb 2x 150 bp
High output, 75 cycles 400 million 30 Gb 2 x 37 bp 1 x 75 bp
High output, 150 cycles 400 million 60 Gb 2x 75 bp 1x 150 bp
High output, 300 cycles 400 million 120 Gb 2x 150 bp